Ultrasensitive Insulin ELISA
£370.00
A high quality enzyme immunoassay for specific quantification of human insulin in serum or plasma.
Kit format: 1 x 96 wells
Measurement range: 0.15 – 20 mU/L (0.0065 – 0.87 µg/L)
- Calibrated against 1st International Reference Preparation 66/304 for human insulin
- No cross-reactivity to C-peptide or Proinsulin des(31-32) or split (32-33)
- Insignificant or no cross-reactivity to insulin analogs
- CE/IVD labeled on select markets
Test Characteristics
Samples: Serum, plasma
Sample volume: 25 µL
Assay range: 0.15 – 20 mU/L
Detection limit: ≤ 0.15 mU/L (0.0065 µg/L)
Incubation: 1 h + 30 min
Detection method: absorbance
Specificity
The following cross-reactions have been tested:
C-peptide <0.01 %
Proinsulin <0.01 %
Proinsulin des (31-32) <0.5 %
Proinsulin split (32-33) <0.5 %
Proinsulin des (64-65) 98 %
Proinsulin split (65-66) 56 %
Insulin aspart 3 %
Insulin detemir n.d.
Insulin glargine 12 %
Insulin glulisine n.d.
Insulin lispro n.d.
IGF-I <0.02 %
IGF-II <0.02 %
Rat insulin 0.7 %
Mouse insulin 0.3 %
Porcine insulin 93 %
Ovine insulin 48 %
Bovine insulin 31 %
n.d.=not detected
