Ultrasensitive Insulin ELISA (10-1132-01)
A high quality enzyme immunoassay for specific quantification of human insulin in serum or plasma.
Kit format: 1 x 96 wells
Measurement range: 0.15 – 20 mU/L (0.0065 – 0.87 µg/L)
Description
- Calibrated against 1st International Reference Preparation 66/304 for human insulin
- No cross-reactivity to C-peptide or Proinsulin des(31-32) or split (32-33)
- Insignificant or no cross-reactivity to insulin analogs
- CE/IVD labeled on select markets
Test Characteristics
Parameter | Value |
Samples | Serum, plasma |
Sample volume | 25 µL |
Assay range | 0.15 – 20 mU/L |
Detection limit | ≤ 0.15 mU/L (0.0065 µg/L) |
Incubation | 1 h + 30 min |
Detection method | Absorbance |
Specificity
Substance | Cross-reactivity (%) |
C-peptide | < 0.01 % |
Proinsulin | < 0.01 % |
Proinsulin des (31-32) | < 0.5 % |
Proinsulin split (32-33) | < 0.5 % |
Proinsulin des (64-65) | 98 % |
Proinsulin split (65-66) | 56 % |
Insulin aspart | 3 % |
Insulin detemir | n.d. |
Insulin glargine | 12 % |
Insulin glulisine | n.d. |
Insulin lispro | n.d. |
IGF-I | < 0.02 % |
IGF-II | < 0.02 % |
Rat insulin | 0.7 % |
Mouse insulin | 0.3 % |
Porcine insulin | 93 % |
Ovine insulin | 48 % |
Bovine insulin | 31 % |