The Mercodia Ultrasensitive C-peptide ELISA is a high quality immunoassay for specific quantification of human C-peptide in serum, plasma, cell culture media or urine.
- High sensitivity, detetction limit < 2.5 pmol/L (0.0076 µg/L)
- No or minimal cross-reactivity to insulin or proinsulin
- Calibrated against International Reference Reagent IRR84/510 for human C-peptide.
- CE/IVD labeled on select markets
Samples: Serum, EDTA and heparin plasma as well as urine.
Sample volume: 50 µL
Assay range: 5 – 280 pmol/L (0.015 – 0.85 ng/mL)
Detection limit: 2.5 pmol/L (0.0076 µg/L)
Incubation: 1 h + 1 h + 30 min
Detection method: absorbance
The following cross-reactions have been tested:
Insulin < 0.0006 %
Proinsulin 5 %
Proinsulin (Des 31-32) 3 %
Proinsulin (Split 32-33) 2 %
Proinsulin (Des 64-65) 74 %
Proinsulin (Split 65-66) 10 %