Ultrasensitive C-peptide ELISA (10-1141-01)

Ultrasensitive C-peptide ELISA (10-1141-01)

The Mercodia Ultrasensitive C-peptide ELISA is a high quality immunoassay for specific quantification of human C-peptide in serum, plasma, cell culture media or urine.

Kit format: 1 x 96 wells
Measurement range: 5 – 280 pmol/L (0.015 – 0.85 ng/mL)

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Description

  • High sensitivity, detetction limit < 2.5 pmol/L (0.0076 µg/L)
  • No or minimal cross-reactivity to insulin or proinsulin
  • Calibrated against International Reference Reagent IRR84/510 for human C-peptide.
  • CE/IVD labeled on select markets

Test Characteristics

Parameter

Value

Samples

Serum, EDTA and heparin plasma, as well as urine

Sample volume

50 µL

Assay range

5 – 280 pmol/L (0.015 – 0.85 ng/mL)

Detection limit

2.5 pmol/L (0.0076 µg/L)

Incubation

1 h + 1 h + 30 min

Detection method

Absorbance

Specificity

Substance

Cross-reactivity (%)

Insulin

< 0.0006 %

Proinsulin

5 %

Proinsulin (Des 31-32)

3 %

Proinsulin (Split 32-33)

2 %

Proinsulin (Des 64-65)

74 %

Proinsulin (Split 65-66)

10 %