Rat Insulin ELISA (10-1250-01/10)
The Rat Insulin ELISA is based on highly specific monoclonal antibodies with insignificant or no cross-reactivity to C-peptide or proinsulin.
Kit format: 1 x 96 wells or 10 x 96 wells
Measurement range: 0.15 – 5.5 μg/L
Description
- Low sample volume – 10 μL (5 µL assay protocol available)
- Insignificant or no cross reactivity to C-peptide or proinsulin
- No interference from complex sample matrices
Test Characteristics
Parameter | Value |
Samples | Serum, EDTA plasma, and cell culture medium |
Sample volume | 10 µL (5 µL protocol available) |
Assay range | 0.15 – 5.5 μg/L |
Detection limit | ≤ 0.15 µg/L |
Incubation | 2 h + 15 min |
Specificity
Substance | Cross-reactivity (%) |
Rat C-peptide | < 0.001 % |
Rat proinsulin | 7 % |
Mouse insulin | 75 % |
Human insulin | 167 % |
Human proinsulin | 75 % |
Human C-peptide | < 0.05 % |
Insulin lispro | 167 % |