Proinsulin ELISA (10-1118-01)
Mercodia Proinsulin ELISA provides a method for the quantitative determination of human proinsulin in serum or plasma.
Kit format: 1 x 96 wells
Measurement range: 3.3 – 130 pmol/L (0.03 – 1.18 µg/L)
Description
- Measures total proinsulin
- Calibrated against International Reference Reagent IRR 84/611 for human proinsulin
- Highly specific with no cross-reactivity to human insulin or C-peptide
Test Characteristics
Parameter | Value |
Samples | Serum and plasma |
Sample volume | 50 µL |
Assay range | 3.3 – 130 pmol/L |
Detection limit | 1.7 pmol/L |
Incubation | 1 h + 1 h + 15 min |
Detection method | Absorbance |
Specificity
Substance | Cross-reactivity (%) |
Insulin | < 0.03 % |
C-peptide | < 0.006 % |
Proinsulin (Des 31-32) | 95 % |
Proinsulin (Split 32-33) | 95 % |
Proinsulin (Des 64-65) | 84 % |
Proinsulin (Split 65-66) | 90 % |
Porcine Proinsulin | 4 % |
Mouse Proinsulin I | 3 % |
Mouse Proinsulin II | 16 % |
Rat Proinsulin I | 13 % |
Rat Proinsulin II | 10 % |