Porcine C-peptide ELISA (10-1256-01)
An assay for the quantitative determination of porcine C-peptide in serum, plasma and cell culture medium.
Kit format: 1 x 96 wells
Measurement range: 20-1200 pmol/L
Description
- No cross reactivity to human proinsulin or insulin
- No cross reactivity to C-peptide in human, macaque, rat or mouse
- High specificity – enables specific measurement of porcine C-peptide in xenotransplantation studies
Test Characteristics
Parameter | Details |
Samples | Serum, plasma, and cell culture medium |
Sample Volume | 10 µL |
Assay Range | 20 – 1200 pmol/L |
Detection Limit | 10 pmol/L |
Incubation | 2 h + 1 h + 15 min |
Detection Method | Absorbance |
Specificity
Analyte | Cross-reactivity |
Porcine insulin | < 0.05% |
Porcine proinsulin | < 0.02% |
Human C-peptide | < 0.003% |
Human insulin | < 0.002% |
Human proinsulin | < 0.01% |
Macaque C-peptide | < 0.006% |
Mouse insulin | < 0.13% |
Mouse proinsulin | < 0.002% |
Rat C-peptide | 0.08% |
Rat insulin | < 0.002% |
Rat proinsulin | < 0.002% |