Iso-Insulin ELISA*

Kit format: 1 x 96 wells
Measurement range: 3 – 100 mU/L (0.13 – 4.35 µg/L)

• Measures total insulin
• Cross-reactivity with most insulin analogs
• Calibrated against 1st International Reference Preparation 66/304 for human insulin.
• CE/IVD labeled on select markets

Test Characteristics

Samples: Serum, plasma, cell culture medium
Sample volume: 25 µL
Assay range: 3 – 100 mU/L
Detection limit: 2 mU/L (0.086 µg/L)
Incubation: 1 h + 15 min
Detection method: absorbance

Specificity

The following cross-reactions have been tested:

Insulin 100 %
C-peptide < 0.1 %
Proinsulin 54 %
Proinsulin des (31-32) 58 %
Proinsulin split (32-33) 56 %
Proinsulin des (64-65) 66 %
Proinsulin split (65-66) 78 %
Insulin lispro 112 %
Insulin aspart 100 %
Insulin determir 28 %
Insulin glargine 58 %
Insulin glulisine 123 %
IGF-I < 0.02 %
IGF-II < 0.02 %
Rat insulin 71 %
Mouse insulin 49 %
Porcine insulin 76 %
Ovine insulin 131 %
Bovine insulin 58 %