Iso-Insulin ELISA (10-1128-01)
Mercodia Iso-Insulin ELISA provides a method for the quantitative determination of total insulin.
Kit format: 1 x 96 wells
Measurement range: 3 – 100 mU/L (0.13 – 4.35 µg/L)
Description
- Measures total insulin
- Cross-reactivity with most insulin analogs
- Calibrated against 1st International Reference Preparation 66/304 for human insulin.
- CE/IVD labeled on select markets
Test Characteristics
Parameter | Value |
Samples | Serum, plasma, cell culture medium |
Sample volume | 25 µL |
Assay range | 3 – 100 mU/L |
Detection limit | 2 mU/L (0.086 µg/L) |
Incubation | 1 h + 15 min |
Detection method | Absorbance |
Specificity
Substance | Cross-reactivity (%) |
Insulin | 100 % |
C-peptide | < 0.1 % |
Proinsulin | 54 % |
Proinsulin des (31-32) | 58 % |
Proinsulin split (32-33) | 56 % |
Proinsulin des (64-65) | 66 % |
Proinsulin split (65-66) | 78 % |
Insulin lispro | 112 % |
Insulin aspart | 100 % |
Insulin detemir | 28 % |
Insulin glargine | 58 % |
Insulin glulisine | 123 % |
IGF-I | < 0.02 % |
IGF-II | < 0.02 % |
Rat insulin | 71 % |
Mouse insulin | 49 % |
Porcine insulin | 76 % |
Ovine insulin | 131 % |
Bovine insulin | 58 % |