Insulin ELISA*

£290.00

A high quality ELISA for specific measurement of human insulin in serum, plasma or cell culture media.

SKU: 10-1113-01 Category:

Kit format: 1 x 96 wells
Measurement range: 3 – 200 mU/L (0.13 – 8.70 µg/L)

  • Calibrated against 1st International Reference Preparation 66/304 for human insulin
  • No cross-reactivity to C-peptide or Proinsulin des(31-32) or split (32-33)
  • Insignificant or no cross-reactivity to insulin analogs
  • CE/IVD labeled on select markets

Test Characteristics Samples: Serum, plasma, cell culture media Sample volume: 25 µL Assay range: 3 – 200 mU/L Detection limit: ≤ 1 mU/L (0.043 µg/L) Incubation: 1 h + 15 min Detection method: absorbance Specificity: The following cross-reactions have been tested: C-peptide <0.01 % Proinsulin <0.01 % Proinsulin des (31-32) <0.5 % Proinsulin split (32-33) <0.5 % Proinsulin des (64-65) 98 % Proinsulin split (65-66) 56 % Insulin aspart 4 % Insulin detemir n.d. Insulin glargin 24 % Insulin glulisine n.d. Insulin lispro n.d. IGF-I <0.02 % IGF-II <0.02 % Rat insulin 0.7 % Mouse insulin 0.3 % Porcine insulin 93 % Ovine insulin 48 % Bovine insulin 31 % n.d.=not detected