Insulin ELISA (10-1113-01/10)
A high quality ELISA for specific measurement of human insulin in serum, plasma or cell culture media.
Kit format: 1 x 96 or 10 x 96 wells
Measurement range: 3 – 200 mU/L (0.13 – 8.70 µg/L)
Description
- Calibrated against 1st International Reference Preparation 66/304 for human insulin
- No cross-reactivity to C-peptide or Proinsulin des(31-32) or split (32-33)
- Insignificant or no cross-reactivity to insulin analogs
- CE/IVD labeled on select markets
Test Characteristics
Parameter | Value |
Samples | Serum, plasma, cell culture media |
Sample volume | 25 µL |
Assay range | 3 – 200 mU/L |
Detection limit | ≤ 1 mU/L (0.043 µg/L) |
Incubation | 1 h + 15 min |
Detection method | Absorbance |
Specificity
Substance | Cross-reactivity (%) |
C-peptide | <0.01 % |
Proinsulin | <0.01 % |
Proinsulin des (31-32) | <0.5 % |
Proinsulin split (32-33) | <0.5 % |
Proinsulin des (64-65) | 98 % |
Proinsulin split (65-66) | 56 % |
Insulin aspart | 4 % |
Insulin detemir | n.d. |
Insulin glargine | 24 % |
Insulin glulisine | n.d. |
Insulin lispro | n.d. |
IGF-I | <0.02 % |
IGF-II | <0.02 % |
Rat insulin | 0.7 % |
Mouse insulin | 0.3 % |
Porcine insulin | 93 % |
Ovine insulin | 48 % |
Bovine insulin | 31 % |