High Range Rat Insulin ELISA

Kit format: 1 x 96 wells
Measurement range: 3 – 150 µg/L

• No sample dilution for sample with high insulin concentrations
• Low sample volume – 10 µL
• Results within 2.5 hours

Test Characteristics

Samples: Serum, EDTA plasma and cell culture medium.
Sample volume: 10 µL
Assay range: 3 – 150 μg/L
Detection limit: ≤ 1.5 µg/L
Incubation: 2 h + 15 min

Specificity

The ELISA kit measures rat insulin to 100%.

Mouse insulin 75%
Rat C-peptide I n.d.
Rat C-peptide II n.d.
Mouse C-peptide I n.d.
Mouse C-peptide II n.d.
Rat Proinsulin I 8%
Rat Proinsulin II 51%
Mouse Proinsulin I 33%
Mouse Proinsulin II 51%

Human C-peptide n.d.
Human insulin 167%
Human proinsulin 75%
Insulin lispro (Humalog® Eli Lilly) 167%

n.d. = not detected