High Range Rat Insulin ELISA (10-1145-01)
Mercodia High Range Rat Insulin ELISA provides a method for the quantitative determination of high levels of insulin in rat samples.
Kit format: 1 x 96 wells
Measurement range: 3 – 150 µg/L
Description
- No sample dilution for sample with high insulin concentrations
- Low sample volume – 10 µL
- Results within 2.5 hours
Test Characteristics
Parameter | Value |
Samples | Serum, EDTA plasma, and cell culture medium |
Sample volume | 10 µL |
Assay range | 3 – 150 μg/L |
Detection limit | ≤ 1.5 µg/L |
Incubation | 2 h + 15 min |
Specificity
Substance | Cross-reactivity (%) |
Rat insulin | 100 % |
Mouse insulin | 75 % |
Rat C-peptide I | N.D. |
Rat C-peptide II | N.D. |
Mouse C-peptide I | N.D. |
Mouse C-peptide II | N.D. |
Rat proinsulin I | 8 % |
Rat proinsulin II | 51 % |
Mouse proinsulin I | 33 % |
Mouse proinsulin II | 51 % |
Human C-peptide | N.D. |
Human insulin | 167 % |
Human proinsulin | 75 % |
Insulin lispro (Humalog® Eli Lilly) | 167 % |