Glucagon 10ul ELISA (10-1281-01)
Mercodia Glucagon ELISA – 10 μL is a highly specific and sensitive immunoassay for measuring glucagon in serum, EDTA plasma, and cell culture media samples from rat, mouse, and non-human primates.
Kit format: 1 x 96 wells
Measurement range: 2-180 pmol/L (7 – 627 pg/mL)
Description
- Low sample volume – 10 µL
- No purification of samples
- Sample types: Serum, EDTA Plasma, and cell-culture medium
- Measurement range: 2 – 180 pmol/L (7 – 627 pg/mL)
- Detection limit: 1.5 pmol/L (5.23 pg/mL)
- Little or no cross-reactivity to oxyntomodulin, glicentin, mini-glucagon, GLP-1, GLP-2 and GRPP
- Highly appreciated and often cited assay
Test Characteristics
Parameter | Value |
Samples | Serum, EDTA plasma, cell culture medium |
Sample volume | 10 µL |
Assay range | 2-180 pmol/L (7 – 627 pg/mL) |
Detection limit | 1.5 pmol/L |
Incubation | 18-22 h (overnight) + 15 min |
Detection method | Absorbance |
Specificity
Substance | Cross-reactivity (%) |
Mini-glucagon | N.D. |
Glicentin, human | 1.0 % |
Glicentin, mouse | 7.0 % |
Glicentin, rat | 4.0 % |
Oxyntomodulin, human/rat/mouse | 2.0 % |
Oxyntomodulin, bovine/canine/porcine | N.D. |
GLP-1 (7-36) | N.D. |
GLP-1 (9-36) | N.D. |
GLP-2 | N.D. |