Mammalian Diabetes

DescriptionRat Insulin ELISAUltrasensitive Rat Insulin ELISAHigh Range Rat Insulin ELISA
Code No.10-1250-01
10-1250-10
10-1251-0110-1145-01
No. of tests96/10x969696
Measured antigenRat/mouse insulinRat/mouse insulinRat/mouse insulin
Standards mU/l0, 0.15, 0.4, 1, 3, 5.50, 0.02, 0.05, 0.15 0.4, 1.00, 3.0, 7.5, 30.0, 75.0, 150.0
Calibration
Incubations (mins)120+15120+15120+15
Sample/sample volumeserum/plasma 10ulserum/plasma 25ulserum/plasma 10ul
Sensitivity≤0.15ug/l1) ≤0.02ug/l1.5 ug/l/
263 pmol/l
Precision intra/inter cv%<5.2%, <10.1%1) <3% 1) <5%<5%/<5%
Hook>450 ug/l450 ug/l576 ug/l
100.8nmol/l
Cross-reactions- Human insulin 167%
- Insulin Lispro (Humalog®) 167%
- Human proinsulin 75%
- Human C-peptide <0.05%
- IGF-I <0.02%
- IGF-II <0.02%
- Rat C-peptide <0.001%
- Rat proinsulin 7%
- Mouse insulin 75%
- Porcine insulin 476%
- Sheep insulin 179%
- Bovine insulin 78%
- No interference by lipaemic, icteric or haemolysed samples
- insulin (mouse) 81%
- insulin (human) 120%
- insulin (Humalog®)120%
- proinsulin (human) 71%
- C-peptide (human) <0.05%
- No interference by lipaemic, icteric or haemolysed samples
- insulin (mouse) 81%
- insulin (human) 120%
- insulin (Humalog®) 120
- proinsulin (human) 71% C-peptide (human)<0.05%
- No interference by lipaemic, icteric or haemolysed samples

DescriptionPorcine Insulin ELISAOvine Insulin ELISABovine Insulin ELISA
Code No.10-1200-0110-1202-0110-1201-01
No. of tests969696
Measured antigenPorcine insulinOvine insulinBovine insulin
Standards mU/l0, 0.02, 0.05, 0.15, 0.5, 1.50, 0.05, 0.15, 0.5, 1.5, 3.00, 0.05, 0.15, 0.5, 1.5, 3.0
Calibrationin-house referencein-house referencein-house reference
Incubations (mins)120+15120+15120+15
Sample/sample volumeSerum/plasma 25ulSerum/plasma 25ulSerum/plasma 25ul
Sensitivity≤0.01ug/l≤0.025ug/l≤0.025ug/l
Precision intra/inter cv%<3.8% <3.9%<3.7% <6.5%<5.3% <7.0%
Hook>1000ug/l>1000ug/l>1000ug/l
Cross-reactionsNo interference by lipaemic, icteric or haemolysed samplesNo interference by lipaemic, icteric or haemolysed samplesNo interference by lipaemic, icteric or haemolysed samples

DescriptionMouse Insulin ELISAUltrasensitive Mouse Insulin ELISAGlucagon 10ul ELISA
Code No.10-1247-01
10-1247-10
10-1249-0110-1281-01
No. of tests96/10x969696
Measured antigenMouse InsulinMouse InsulinGlucagon. validated for rat, mice, porcine & non-human primate.
Standards mU/l0, 0.188, 0.5, 1.25, 3.75, 6.90, 0.025, 0.063, 0.188, 0.5, 1.25
Incubations (mins)120+15120+15overnight+15
Sample/sample volumeserum/plasma 10ulserum/plasma 25ulSerum/plasma/cell culture
Sensitivity≤0.2 ug/l≤0.025 ug/l1.5 pmol/l
Precision intra/inter cv%<3.2%/<6%<3%/<5%Total 8.3 17.9%
Hook>450 ug/l450 ug/l>8 umol/l
Cross-reactions- Human insulin 167%
No interference by lipaemic, icteric or haemolysed samples
No interference by lipaemic, icteric or haemolysed samples- Oxyntomodulin, human, rat, mouse 2.0%
- Oxyntomodulin, bovine, canine, porcine n.d.
- Mini-Glucagon n.d.
- GLP-1 n.d.
- GLP-2 n.d.
- Glicentin, human 1.0%
- Glicentin, mouse 7.0%
- Glicentin, rat 4.0%

DescriptionRat C-peptide ELISARat/Mouse proinsulin ELISA
Code No.10-1172-0110-1232-01
No. of tests9696
Measured antigenRat C-peptideRat and Mouse proinsulin
Standards mU/lc. 0 - 4000 (0 + 5 standards)c. 0 - 2000 (0 + 5 standards)
Incubations (mins)60 + 60 + 15120+30
Sample/sample volumeserum/plasma 10ulserum/plasma 25ul
Sensitivity%td 3 pmol/l
Precision intra/inter cv%<4.3%/<7.3%<3.7%/<6.4%
Hook400000pmol/l90000pmol/l
Cross-reactions- Human C-peptide <0.001%
- Rat proinsulin 4.55%
- Rat insulin <0.01%
- No interference by lipaemic, icteric or haemolysed samples
- Human insulin <0.0000001%
- Human proinsulin 2 - 10% (mean 5%)
- Human C-peptide <0.000001%
- Rat C-peptide <0.0000001%
- Rat insulin <0.000004%
- Mouse insulin <0.0003%
- Mouse C-peptide <0.00007%
- No interference by lipaemic, icteric or haemolysed samples

DescriptionPorcine C-peptide ELISAFeline Insulin ELISA
Code No.10-1156-0110-1233-01
No. of tests9696
Measured antigenPorcine C-peptideFeline Insulin
Standards mU/lc. 0—1500 (0 + 5 standards)0, 5, 35, 100, 350, 700 ng/l
Incubations (mins)120 + 60 + 15120+30
Sample/sample volumeserum/plasma/cell culture 10ulserum/plasma 10ul
Sensitivity10 pmol/L5ng/l
Precision intra/inter cv%<3.7%/<3.8%<4.8%/<8.9%
Hook>30000 pmol/L27600 ug/l
Cross-reactions- Porcine insulin <0.05%
- Porcine proinsulin <0.02%
- Human C-peptide <0.003%
- Human proinsulin <0.01%
- Macaque C-peptide <0.006%
- Mouse C-peptide <0.007%
- Mouse insulin <0.13%
- Mouse proinsulin <0.002%
- Rat C-peptide 0.08%
- Rat insulin <0.002%
- Rat proinsulin <0.002%
- Aspart (NovoRapid®) 5.9%
- Detemir (Levemir®) <0.008%
- Glargin (Lantus®) 8.4%
- Lispro (Humalog®) <0.00000002%
- Glulisine (Apidra®) <0.0000007%
- Letsulin® , Caninsulin® 57.4%
- No interference by lipaemic, icteric or haemolysed samples

DescriptionCanine Insulin ELISAEquine Insulin ELISA
Code No.10-1203-0110-1205-01
No. of tests9696
Measured antigenCanine InsulinEquine Insulin
Standards mU/l0, 0.02, 0.05, 0.15, 0.50, 1.500, 0.02, 0.05, 0.15, 0.50, 1.50
Incubations (mins)120 + 15120 + 15
Sample/sample volumeserum/plasma 25ulserum/plasma 25ul
Sensitivity≤0.01 ug/l≤0.01 ug/l
Precision intra/inter cv%<4.5%/<6.2%<3.4%/<4.3%
Hook>1000ug/l>1000ug/l
Cross-reactions- porcine insulin 100%
- porcine C-peptide <0.001%
- porcine proinsulin <0.20%
- human insulin 28%
- human C-peptide <0.01%
- human proinsulin <0.1%
- NovoRapid 0.7%
- Levemir <0.0000002%
- Lantus 5.4%
- Humalog <0.0000006%
- No interference by lipaemic, icteric or haemolysed samples
- porcine insulin 100%
- porcine C-peptide <0.001%
- porcine proinsulin <0.20%
- human insulin 28%
- human C-peptide <0.01%
- human proinsulin <0.1%
- NovoRapid 0.7%
- Levemir <0.0000002%
- Lantus 5.4%
- Humalog <0.0000006%
- No interference by lipaemic, icteric or haemolysed samples