Mammalian Diabetes

The Rat Insulin ELISA is based on highly specific monoclonal antibodies with insignificant or no cross-reactivity to C-peptide or proinsulin.

Kit format: 1 x 96 wells or 10 x 96 wells
Measurement range: 0.15 – 5.5 μg/L

  • Low sample volume – 10 μL (5 µL assay protocol available)
  • Insignificant or no cross reactivity to C-peptide or proinsulin
  • No interference from complex sample matrices

Test Characteristics

  • Samples: Serum, EDTA plasma and cell culture medium.
  • Sample volume: 10 µL (5 µL protocol available)
  • Assay range: 0.15 – 5.5 μg/L
  • Detection limit: ≤ 0.15 µg/L
  • Incubation: 2 h+ 15 min

Specificity

  • Rat C-peptide < 0.001%
  • Rat proinsulin 7%
  • Mouse insulin 75%
  • Human insulin 167%
  • Human proinsulin 75%
  • Human C-peptide < 0.05%
  • Insulin lispro 167%

Mercodia Ultrasensitive Rat Insulin ELISA provides a method for the quantitative determination of low levels of insulin in rat samples.

Kit format: 1 x 96 wells
Measurement Range: 0.025 – 1.5µg/L

  • Very low detection limit – 0.02 µg/L
  • High specificity – Insignificant cross-reactivity to C-peptide and proinsulin
  • No interference from complex sample matrices

Test Characteristics

  • Samples: Serum, EDTA plasma and cell culture medium.
  • Sample volume: 25 µL
  • Assay range: 0.02 – 1.0 μg/L
  • Detection limit: ≤ 0.02 µg/L
  • Incubation: 2 h+ 30 min

Specificity

  • The ELISA kit measures rat insulin to 100%.
  • Mouse insulin 75 %
  • Rat proinsulin I 8 %
  • Rat proinsulin II 51 %
  • Mouse proinsulin I 31 %
  • Mouse proinsulin II 51 %
  • Mouse c-peptide I n.d.
  • Mouse c-peptide II n.d.
  • Rat c-peptide I n.d.
  • Rat c-peptide II n.d.
  • Human insulin 167 %
  • Human proinsulin 75 %
  • Human C-peptide n.d.
  • Insulin lispro (Humalog®) 167 %

Mercodia High Range Rat Insulin ELISA provides a method for the quantitative determination of high levels of insulin in rat samples.

Kit format: 1 x 96 wells
Measurement range: 3 – 150 µg/L

  • No sample dilution for sample with high insulin concentrations
  • Low sample volume – 10 µL
  • Results within 2.5 hours

Test Characteristics

  • Samples: Serum, EDTA plasma and cell culture medium.
  • Sample volume: 10 µL
  • Assay range: 3 – 150 μg/L
  • Detection limit: ≤ 1.5 µg/L
  • Incubation: 2 h + 15 min

Specificity

  • The ELISA kit measures rat insulin to 100%.
  • Mouse insulin 75%
  • Rat C-peptide I n.d.
  • Rat C-peptide II n.d.
  • Mouse C-peptide I n.d.
  • Mouse C-peptide II n.d.
  • Rat Proinsulin I 8%
  • Rat Proinsulin II 51%
  • Mouse Proinsulin I 33%
  • Mouse Proinsulin II 51%
  • Human C-peptide n.d.
  • Human insulin 167%
  • Human proinsulin 75%
  • Insulin lispro (Humalog® Eli Lilly) 167%

Immunoassay for quantitative determination of rat C-peptide in serum, plasma or cell culture medium.

Kit format: 1 x 96 wells
Measurement range: 100 – 4000 pmol/L (0.33 – 13 µg/L)

  • Low sample volume – 10μL
  • Measures both Rat C-peptide I and II
  • Minimal cross-reactivity to human or mouse insulin, C-peptide or proinsulin

Test Characteristics

  • Samples: serum,EDTA plasma or cell culture media
  • Sample volume: 10 µL
  • Assay range: 100 – 4000 pmol/L (0.33 – 13 µg/L)
  • Detection limit: ≤ 100 pmol/L (0.33 μg/L)
  • Incubation: 1h + 1h + 15 min
  • Detection method: absorbance

Specificity

  • Rat insulin n.d.
  • Rat proinsulin 4.6 %
  • Mouse C-peptide 6 %
  • Porcine C-peptide n.d.
  • Human C-peptide n.d.

ELISA kit for quantitative determination of rat and mouse proinsulin in serum, plasma, cell culture media or tissue homogenate.

Kit format: 1 x 96 wells
Measurement range: 3.5 – 200 pmol/L

  • Specific for rat and mouse proinsulin I and II
  • No cross reactivity to insulin or C-peptide
  • Fast and easy-to-use

Test Characteristics

  • Samples: Serum, plasma, cell culture medium, tissue homogenate
  • Sample volume: 25 µL
  • Assay range: 3.5 – 200 pmol/L
  • Detection limit: ≤ 3 pmol/L
  • Incubation: 120 + 30 min

Specificity

  • Rat proinsulin I 100%
  • Rat proinsulin II 145 %
  • Rat insulin n.d.
  • Rat C-peptide n.d.
  • Mouse insulin n.d.
  • Mouse C-peptide n.d.
  • Mouse proinsulin I 234 %
  • Mouse proinsulin II 100 %
  • Human insulin n.d.
  • Human C-peptide n.d.
  • Human proinsulin 0.5 %

The Mercodia Mouse Insulin ELISA is an easy-to-use assay based on highly specific monoclonal antibodies with insignificant or no cross-reactivity to C-peptide or proinsulin.

Kit format: 1 x 96 wells or 10 x 96 wells
Measurement range: 0.2 – 6.5 µg/L

  • Low sample volume – 10µL (5µL assay protocol available)
  • Easy assay protocol (Shortened – 35 min – assay protocol available)
  • Insignificant or no cross-reactivity to mouse C-peptide or proinsulin

Test Characteristics

  • Samples: Serum, EDTA plasma and cell culture medium.
  • Sample volume: 10 µL (5 µL protocol available)
  • Assay range: 0.2 – 6.5 µg/L
  • Detection limit: ≤ 0.2 µg/L
  • Incubation: 120 + 15 min

Specificity

  • Mouse C-peptide I n.d.
  • Mouse C-peptide II n.d.
  • Rat C-peptide I n.d.
  • Rat C-peptide II n.d.
  • Rat insulin 146 %
  • Mouse proinsulin I 43 %
  • Mouse proinsulin II 60 %
  • Rat proinsulin I 14 %
  • Rat proinsulin II 60 %
  • Human insulin 195 %
  • Human proinsulin 82 %
  • Human C-peptide n.d

Mercodia Ultrasensitive Rat Insulin ELISA provides a method for the quantitative determination of low levels of insulin in rat samples.

Kit format: 1 x 96 wells
Measurement range: 0.025 – 1.5 μg/L

  • Very low detection limit – 0.025 µg/L
  • High specificity – Insignificant cross-reactivity to C-peptide and proinsulin
  • No interference from complex sample matrices

Test Characteristics

  • Samples: Serum, EDTA plasma and cell culture medium.
  • Sample volume: 25 µL
  • Assay range: 0.025 – 1.0 μg/L
  • Detection limit: ≤ 0.025 µg/L
  • Incubation: 120 + 30 min

Specificity

  • Rat insulin 146%
  • Rat proinsulin 14%
  • Rat C-peptide n.d.
  • Human insulin 195%
  • Human proinsulin 82%
  • Human C-peptide n.d.

Mercodia Glucagon ELISA – 10 μL is a highly specific and sensitive immunoassay for measuring glucagon in serum, EDTA plasma, and cell culture media samples from rat, mouse, and non-human primates.

Kit format: 1 x 96 wells
Measurement range: 2-180 pmol/L (7 – 627 pg/mL)

  • Low sample volume – 10 µL
  • No purification of samples
  • Sample types: Serum, EDTA Plasma, and cell-culture medium
  • Measurement range: 2 – 180 pmol/L (7 – 627 pg/mL)
  • Detection limit: 1.5 pmol/L (5.23 pg/mL)
  • Little or no cross-reactivity to oxyntomodulin, glicentin, mini-glucagon, GLP-1, GLP-2 and GRPP
  • Highly appreciated and often cited assay

Test Characteristics

  • Samples: Serum, EDTA plasma and cell culture medium.
  • Sample volume: 10 µL
  • Assay range: 2-180 pmol/L (7 – 627 pg/mL)
  • Detection limit: 1.5 pmol/L
  • Incubation: 18-22 h (overnight) + 15 min
  • Detection method: absorbance

Specificity

  • Mini-glucagon, n.d.
  • Glicentin, human 1.0 %
  • Glicentin, mouse 7.0 %
  • Glicentin, rat 4.0 %
  • Oxyntomodulin, human/rat/mouse 2.0 %
  • Oxyntomodulin, bovine/canine/porcine n.d.
  • GLP-1 (7-36), n.d.
  • GLP-1 (9-36), n.d.
  • GLP-2, n.d.

The Mercodia Feline Insulin ELISA is a species-optimized, high quality enzyme immunoassay for the quantification of cat insulin in serum or plasma.

Kit format: 1 x 96 wells
Measurement range: 10 – 700 ng/L

  • Specially optimized for feline samples
  • Highly specific – no cross-reactivity to proinsulin or C-peptide
  • High sensitivity – possible to detect minute changes in circulating insulin in felines

Test Characteristics

  • Samples: Serum and plasma
  • Sample volume: 10 µL
  • Assay range: 10 – 700 ng/L
  • Detection limit: ≤ 9.2 ng/L
  • Incubation: 2 h + 15 min
  • Detection method: absorbance

Specificity

  • NovoRapid® (Insulin aspart) 5.9 %
  • Levemir® (Insulin detemir) < 0.008 %
  • Lantus® (Insulin glargin) 8.4 %
  • Humalog® (Insulin lispro) n.d.
  • Apidra® (Insulin glulisine) n.d.
  • Vetsulin®, Caninsulin®

The Mercodia Canine Insulin ELISA is a species-optimized, high quality enzyme immunoassay for the quantification of canine insulin in serum or plasma.

Kit format: 1 x 96 wells
Measurement range: 2.3 – 173 mU/L

  • Specially optimized for canine samples
  • Highly specific, no cross-reactivity to proinsulin, C-peptide or insulin analogs

Test Characteristics

  • Samples: Serum and plasma
  • Sample volume: 25 µL
  • Assay range: 2.3 – 173 mU/L
  • Detection limit: 1.15 mU/L
  • Incubation: 2 h + 15 min
  • Detection method: absorbance

Specificity

  • Porcine Insulin 100 %
  • Porcine C-peptide < 0.001 %
  • Porcine proinsulin < 0.2 %
  • Equine insulin 100 %
  • Human insulin 108 %
  • Human C-peptide < 0.01 %
  • Human proinsulin < 0.1 %
  • NovoRapid 3.2 %
  • Levemir n.d.
  • Lantus 27 %
  • Humalog n.d.

The Mercodia Porcine Insulin ELISA is a species-optimized, high quality enzyme immunoassay for the quantification of porcine insulin in serum or plasma.

Kit format: 1 x 96 wells
Measurement Range: 2.3 – 173 mU/L

  • Specially optimized for porcine samples
  • Highly specific, no cross-reactivity to proinsulin or C-peptide

Test Characteristics

  • Samples: Serum or plasma
  • Sample volume: 25 µL
  • Assay range: 2.3 – 173 mU/L
  • Detection limit: 1.15 mU/L
  • Incubation: 2h + 15 min
  • Detection method: absorbance

Specificity

  • Porcine C-peptide < 0.001 %
  • Porcine proinsulin < 0.2 %
  • Equine insulin 100 %
  • Canine insulin 100 %
  • Human insulin 108 %
  • Human C-peptide < 0.01 %
  • Human proinsulin < 0.1 %
  • NovoRapid 3.2 %
  • Levemir n.d.
  • Lantus 27 %
  • Humalog n.d.

The Mercodia Ovine Insulin ELISA is a species-optimized, high quality enzyme immunoassay for the quantification of sheep insulin in serum or plasma.

Kit format: 1 x 96 wells
Measurement Range: 0.05 – 3.0 μg/L

  • Specifically optimised for ovine samples
  • Highly specific, no cross-reactivity to proinsulin or C-peptide
  • High sensitivity

Test Characteristics

  • Samples: Serum or plasma
  • Sample volume: 25 µL
  • Assay range: 0.05 – 3.0 μg/L
  • Detection limit: 0.025 µg/L
  • Incubation: 2 h + 15 min
  • Detection method: absorbance

Specificity

  • NovoRapid 2.0 %
  • Levemir n.d.
  • Lantus 21 %
  • Humalog n.d.

The Mercodia Equine Insulin ELISA is a species-optimized, high quality enzyme immunoassay for the quantification of equine insulin in serum or plasma.

Kit format: 1 x 96 wells
Measurement range: 2.3 – 173 mU/L

  • Specially optimized for equine samples
  • Useful tool for laminitis investigations

Test Characteristics

  • Samples: Serum, EDTA, heparin and citrate plasma
  • Sample volume: 25 µL
  • Assay range: 2.3 – 173 mU/L
  • Detection limit: 1.15 mU/L
  • Incubation: 2 h + 15 min
  • Detection method: absorbance

Specificity

  • Porcine Insulin 100 %
  • Porcine C-peptide < 0.001 %
  • Porcine Proinsulin < 0.2 %
  • Human Insulin 108 %
  • Human C-peptide < 0.01 %
  • Human Proinsulin < 0.1 %
  • NovoRapid 3.2 %
  • Levemir n.d.
  • Lantus 27 %
  • Humalog n.d.

The Mercodia Bovine Insulin ELISA is a high quality, species-optimized enzyme immunoassay for the quantification of bovine insulin in serum or plasma.

Kit format: 1 x 96 wells
Measurement range: 0.05 – 3.0 μg/L

  • Specially optimized for bovine samples.
  • Highly specific, no cross-reactivity to proinsulin or C-peptide
  • High sensitivity

Test Characteristics

  • Samples: Serum or plasma
  • Sample volume: 25 µL
  • Assay range: 0.05 – 3.0 μg/L
  • Detection limit: 0.025 µg/L
  • Incubation: 2 h + 15 min
  • Detection method: absorbance

Specificity

  • NovoRapid 3.8 %
  • Levemir < 0.0000004 %
  • Lantus 28 %
  • Humalog < 0.000001 %

An assay for the quantitative determination of porcine C-peptide in serum, plasma and cell culture medium.

Kit format: 1 x 96 wells
Measurement range: 20-1200 pmol/L

  • No cross reactivity to human proinsulin or insulin
  • No cross reactivity to C-peptide in human, macaque, rat or mouse
  • High specificity – enables specific measurement of porcine C-peptide in xenotransplantation studies

Test Characteristics

  • Samples: Serum, plasma and cell culture medium
  • Sample volume: 10 µL
  • Assay range: 20-1200 pmol/L
  • Detection limit: 10 pmol/L
  • Incubation: 2 h + 1 h + 15 min
  • Detection method: absorbance

Specificity

  • Porcine insulin < 0.05 %
  • Porcine proinsulin < 0.02 %
  • Human C-peptide < 0.003 %
  • Human insulin < 0.002 %
  • Human proinsulin < 0.01 %
  • Macaque C-peptide < 0.006 %
  • Mouse insulin < 0.13 %
  • Mouse proinsulin < 0.002 %
  • Rat C-peptide 0.08 %
  • Rat insulin < 0.002 %
  • Rat proinsulin < 0.002 %