• Low sample volume – 10 μL (5 µL assay protocol available)
• Insignificant or no cross reactivity to C-peptide or proinsulin
• No interference from complex sample matrices

Mercodia Ultrasensitive Rat Insulin ELISA provides a method for the quantitative determination of low levels of insulin in rat samples.

Kit format: 1 x 96 wells
Measurement Range: 0.025 – 1.5µg/L

• Very low detection limit – 0.02 µg/L
• High specificity – Insignificant cross-reactivity to C-peptide and proinsulin
• No interference from complex sample matrices

Test Characteristics

Samples: Serum, EDTA plasma and cell culture medium.
Sample volume: 25 µL
Assay range: 0.02 – 1.0 μg/L
Detection limit: ≤ 0.02 µg/L
Incubation: 2 h+ 30 min

The ELISA kit measures rat insulin to 100%.
Mouse insulin 75 %
Rat proinsulin I 8 %
Rat proinsulin II 51 %
Mouse proinsulin I 31 %
Mouse proinsulin II 51 %
Mouse c-peptide I n.d.
Mouse c-peptide II n.d.
Rat c-peptide I n.d.
Rat c-peptide II n.d.
Human insulin 167 %
Human proinsulin 75 %
Human C-peptide n.d.
Insulin lispro (Humalog®) 167 %

Directions for use.

Mercodia High Range Rat Insulin ELISA provides a method for the quantitative determination of high levels of insulin in rat samples.

Kit format: 1 x 96 wells
Measurement range: 3 – 150 µg/L

• No sample dilution for sample with high insulin concentrations
• Low sample volume – 10 µL
• Results within 2.5 hours

Test Characteristics

Samples: Serum, EDTA plasma and cell culture medium.
Sample volume: 10 µL
Assay range: 3 – 150 μg/L
Detection limit: ≤ 1.5 µg/L
Incubation: 2 h + 15 min

Specificity

The ELISA kit measures rat insulin to 100%.

Mouse insulin 75%
Rat C-peptide I n.d.
Rat C-peptide II n.d.
Mouse C-peptide I n.d.
Mouse C-peptide II n.d.
Rat Proinsulin I 8%
Rat Proinsulin II 51%
Mouse Proinsulin I 33%
Mouse Proinsulin II 51%

Human C-peptide n.d.
Human insulin 167%
Human proinsulin 75%
Insulin lispro (Humalog® Eli Lilly) 167%

n.d. = not detected

Directions for use.

Immunoassay for quantitative determination of rat C-peptide in serum, plasma or cell culture medium.

Kit format: 1 x 96 wells
Measurement range: 100 – 4000 pmol/L (0.33 – 13 µg/L)

• Low sample volume – 10μL
• Measures both Rat C-peptide I and II
• Minimal cross-reactivity to human or mouse insulin, C-peptide or proinsulin

Test Characteristics

Samples: serum,EDTA plasma or cell culture media
Sample volume: 10 µL
Assay range: 100 – 4000 pmol/L (0.33 – 13 µg/L)
Detection limit: ≤ 100 pmol/L (0.33 μg/L)
Incubation: 1h + 1h + 15 min
Detection method: absorbance

Specificity

The ELISA kit measures both Rat C-peptide I and Rat C-peptide II.

The following cross-reactions have been tested:
Rat insulin n.d.
Rat proinsulin 4.6 %
Mouse C-peptide 6 %
Porcine C-peptide n.d.
Human C-peptide n.d.

n.d. = not detectable

Directions for use.

ELISA kit for quantitative determination of rat and mouse proinsulin in serum, plasma, cell culture media or tissue homogenate.

Kit format: 1 x 96 wells
Measurement range: 3.5 – 200 pmol/L

• Specific for rat and mouse proinsulin I and II
• No cross reactivity to insulin or C-peptide
• Fast and easy-to-use

Test Characteristics

Rat proinsulin I 100%
Rat proinsulin II 145 %

Rat insulin n.d.
Rat C-peptide n.d.
Mouse insulin n.d.
Mouse C-peptide n.d.
Mouse proinsulin I 234 %
Mouse proinsulin II 100 %

Human insulin n.d.
Human C-peptide n.d.
Human proinsulin 0.5 %

n.d. = not detectable

Direction for use.

Mercodia Glucagon ELISA – 10 μL is a highly specific and sensitive immunoassay for measuring glucagon in serum, EDTA plasma, and cell culture media samples from rat, mouse, and non-human primates.

Kit format: 1 x 96 wells
Measurement range: 2-180 pmol/L (7 – 627 pg/mL)

• Low sample volume – 10 µL
• No purification of samples
• Sample types: Serum, EDTA Plasma, and cell-culture medium
• Measurement range: 2 – 180 pmol/L (7 – 627 pg/mL)
• Detection limit: 1.5 pmol/L (5.23 pg/mL)
• Little or no cross-reactivity to oxyntomodulin, glicentin, mini-glucagon, GLP-1, GLP-2 and GRPP
• Highly appreciated and often cited assay

Test Characteristics

Samples: Serum, EDTA plasma and cell culture medium.
Sample volume: 10 µL
Assay range: 2-180 pmol/L (7 – 627 pg/mL)
Detection limit: 1.5 pmol/L
Incubation: 18-22 h (overnight) + 15 min
Detection method: absorbance

Specificity

Mini-glucagon, n.d.
Glicentin, human 1.0 %
Glicentin, mouse 7.0 %
Glicentin, rat 4.0 %
Oxyntomodulin, human/rat/mouse 2.0 %
Oxyntomodulin, bovine/canine/porcine n.d.
GLP-1 (7-36), n.d.
GLP-1 (9-36), n.d.
GLP-2, n.d.

n.d.= not detected

Directions for use.

The Mercodia Feline Insulin ELISA is a species-optimized, high quality enzyme immunoassay for the quantification of cat insulin in serum or plasma.

Kit format: 1 x 96 wells
Measurement range: 10 – 700 ng/L

• Specially optimized for feline samples
• Highly specific – no cross-reactivity to proinsulin or C-peptide
• High sensitivity – possible to detect minute changes in circulating insulin in felines

Test Characteristics

Samples: Serum and plasma
Sample volume: 10 µL
Assay range: 10 – 700 ng/L
Detection limit: ≤ 9.2 ng/L
Incubation: 2 h + 15 min
Detection method: absorbance

Specificity

The following cross-reactions have been tested:

NovoRapid® (Insulin aspart) 5.9 %
Levemir® (Insulin detemir) < 0.008 %
Lantus® (Insulin glargin) 8.4 %
Humalog® (Insulin lispro) n.d.
Apidra® (Insulin glulisine) n.d.
Vetsulin®, Caninsulin®

Directions for use.

The Mercodia Canine Insulin ELISA is a species-optimized, high quality enzyme immunoassay for the quantification of canine insulin in serum or plasma.

Kit format: 1 x 96 wells
Measurement range: 2.3 – 173 mU/L

• Specially optimized for canine samples
• Highly specific, no cross-reactivity to proinsulin, C-peptide or insulin analogs

Test Characteristics

Samples: Serum and plasma
Sample volume: 25 µL
Assay range: 2.3 – 173 mU/L
Detection limit: 1.15 mU/L
Incubation: 2 h + 15 min
Detection method: absorbance

Specificity

The following cross-reactions have been found:

Porcine Insulin 100 %
Porcine C-peptide < 0.001 %
Porcine proinsulin < 0.2 %
Equine insulin 100 %
Human insulin 108 %
Human C-peptide < 0.01 %
Human proinsulin < 0.1 %
NovoRapid 3.2 %
Levemir n.d.
Lantus 27 %
Humalog n.d.
n.d.=not detected

Directions for use.

The Mercodia Porcine Insulin ELISA is a species-optimized, high quality enzyme immunoassay for the quantification of porcine insulin in serum or plasma.

Kit format: 1 x 96 wells
Measurement Range: 2.3 – 173 mU/L

• Specially optimized for porcine samples
• Highly specific, no cross-reactivity to proinsulin or C-peptide

Test Characteristics

Samples: Serum or plasma
Sample volume: 25 µL
Assay range: 2.3 – 173 mU/L
Detection limit: 1.15 mU/L
Incubation: 2h + 15 min
Detection method: absorbance

Specificity

The following cross-reactions have been tested:

Porcine C-peptide < 0.001 %
Porcine proinsulin < 0.2 %
Equine insulin 100 %
Canine insulin 100 %
Human insulin 108 %
Human C-peptide < 0.01 %
Human proinsulin < 0.1 %
NovoRapid 3.2 %
Levemir n.d.
Lantus 27 %
Humalog n.d.
n.d.= not detected

Directions for use.

The Mercodia Ovine Insulin ELISA is a species-optimized, high quality enzyme immunoassay for the quantification of sheep insulin in serum or plasma.

Kit format: 1 x 96 wells
Measurement Range: 0.05 – 3.0 μg/L

• Specifically optimised for ovine samples
• Highly specific, no cross-reactivity to proinsulin or C-peptide
• High sensitivity

Test Characteristics

Samples: Serum or plasma
Sample volume: 25 µL
Assay range: 0.05 – 3.0 μg/L
Detection limit: 0.025 µg/L
Incubation: 2 h + 15 min
Detection method: absorbance

Specificity

The following cross-reactions have been tested:

NovoRapid 2.0 %
Levemir n.d.
Lantus 21 %
Humalog n.d.
n.d.= not detected

Directions for use.

The Mercodia Equine Insulin ELISA is a species-optimized, high quality enzyme immunoassay for the quantification of equine insulin in serum or plasma.

Kit format: 1 x 96 wells
Measurement range: 2.3 – 173 mU/L

• Specially optimized for equine samples
• Useful tool for laminitis investigations

Test Characteristics

Samples: Serum, EDTA, heparin and citrate plasma
Sample volume: 25 µL
Assay range: 2.3 – 173 mU/L
Detection limit: 1.15 mU/L
Incubation: 2 h + 15 min
Detection method: absorbance

Specificity

The following cross-reactions have been found:

Porcine Insulin 100 %
Porcine C-peptide < 0.001 %
Porcine Proinsulin < 0.2 %
Human Insulin 108 %
Human C-peptide < 0.01 %
Human Proinsulin < 0.1 %
NovoRapid 3.2 %
Levemir n.d.
Lantus 27 %
Humalog n.d.

Directions for use.

The Mercodia Bovine Insulin ELISA is a high quality, species-optimized enzyme immunoassay for the quantification of bovine insulin in serum or plasma.

Kit format: 1 x 96 wells
Measurement range: 0.05 – 3.0 μg/L

• Specially optimized for bovine samples.
• Highly specific, no cross-reactivity to proinsulin or C-peptide
• High sensitivity

Test Characteristics

Samples: Serum or plasma
Sample volume: 25 µL
Assay range: 0.05 – 3.0 μg/L
Detection limit: 0.025 µg/L
Incubation: 2 h + 15 min
Detection method: absorbance

Specificity

The following cross-reactions have been tested:

NovoRapid 3.8 %
Levemir < 0.0000004 %
Lantus 28 %
Humalog < 0.000001 %

Directions for use.

An assay for the quantitative determination of porcine C-peptide in serum, plasma and cell culture medium.

Kit format: 1 x 96 wells
Measurement range: 20-1200 pmol/L

• No cross reactivity to human proinsulin or insulin
• No cross reactivity to C-peptide in human, macaque, rat or mouse
• High specificity – enables specific measurement of porcine C-peptide in xenotransplantation studies

Test Characteristics

Samples: Serum, plasma and cell culture medium
Sample volume: 10 µL
Assay range: 20-1200 pmol/L
Detection limit: 10 pmol/L
Incubation: 2 h + 1 h + 15 min
Detection method: absorbance

Specificity

The following cross-reactions have been tested:

Porcine insulin < 0.05 %
Porcine proinsulin < 0.02 %
Human C-peptide < 0.003 %
Human insulin < 0.002 %
Human proinsulin < 0.01 %
Macaque C-peptide < 0.006 %
Mouse insulin < 0.13 %
Mouse proinsulin < 0.002 %
Rat C-peptide 0.08 %
Rat insulin < 0.002 %
Rat proinsulin < 0.002 %

Directions for use.

Mercodia Animal Insulin Control (Low, Medium, High) is designed to be used as a three-level control for Mercodia Porcine, Bovine, Ovine, Canine and Equine Insulin ELISA kits.

Kit format: 3 x 0.5 mL
Measurement range: low, medium, high

• Easy to transport – Lyophilized serum
• Easy-to-use – Reconstituted in redistilled water

Directions for use.

Control set containing rat and mouse insulin and rat C-peptide to be used in the rat and mouse insulin kits as well as in Rat C-peptide ELISA.

Kit format: 3 x 0.5 mL
Measurement range: low, medium, high

• Easy transport – Lyophilized serum
• Easy-to-use – Reconstituted in redistilled water

Test Characteristics

Perform the Mercodia ELISA according to the Directions for Use handling Diabetes Antigen Control Rat&Mouse (Low, Medium, High) as samples.

Specificity

The controls contain rat and mouse insulin and rat C-peptide.

Directions for use.