Human Diabetes

A high quality ELISA for specific measurement of human insulin in serum, plasma or cell culture media.

Kit format: 1 x 96 or 10 x 96 wells
Measurement range: 3 – 200 mU/L (0.13 – 8.70 µg/L)

  • Calibrated against 1st International Reference Preparation 66/304 for human insulin
  • No cross-reactivity to C-peptide or Proinsulin des(31-32) or split (32-33)
  • Insignificant or no cross-reactivity to insulin analogs
  • CE/IVD labeled on select markets

Test Characteristics

  • Samples: Serum, plasma, cell culture media
  • Sample volume: 25 µL
  • Assay range: 3 – 200 mU/L
  • Detection limit: ≤ 1 mU/L (0.043 µg/L)
  • Incubation: 1 h + 15 min
  • Detection method: absorbance

Specificity

  • C-peptide <0.01 %
  • Proinsulin <0.01 %
  • Proinsulin des (31-32) <0.5 %
  • Proinsulin split (32-33) <0.5 %
  • Proinsulin des (64-65) 98 %
  • Proinsulin split (65-66) 56 %
  • Insulin aspart 4 %
  • Insulin detemir n.d.
  • Insulin glargin 24 %
  • Insulin glulisine n.d.
  • Insulin lispro n.d.
  • IGF-I <0.02 %
  • IGF-II <0.02 %
  • Rat insulin 0.7 %
  • Mouse insulin 0.3 %
  • Porcine insulin 93 %
  • Ovine insulin 48 %
  • Bovine insulin 31 %

Mercodia Iso-Insulin ELISA provides a method for the quantitative determination of total insulin.

Kit format: 1 x 96 wells
Measurement range: 3 – 100 mU/L (0.13 – 4.35 µg/L)

  • Measures total insulin
  • Cross-reactivity with most insulin analogs
  • Calibrated against 1st International Reference Preparation 66/304 for human insulin
  • CE/IVD labeled on select markets

Test Characteristics

  • Samples: Serum, plasma, cell culture medium
  • Sample volume: 25 µL
  • Assay range: 3 – 100 mU/L
  • Detection limit: 2 mU/L (0.086 µg/L)
  • Incubation: 1 h + 15 min
  • Detection method: absorbance

Specificity

  • Insulin 100 %
  • C-peptide < 0.1 %
  • Proinsulin 54 %
  • Proinsulin des (31-32) 58 %
  • Proinsulin split (32-33) 56 %
  • Proinsulin des (64-65) 66 %
  • Proinsulin split (65-66) 78 %
  • Insulin lispro 112 %
  • Insulin aspart 100 %
  • Insulin determir 28 %
  • Insulin glargine 58 %
  • Insulin glulisine 123 %
  • IGF-I < 0.02 %
  • IGF-II < 0.02 %
  • Rat insulin 71 %
  • Mouse insulin 49 %
  • Porcine insulin 76 %
  • Ovine insulin 131 %
  • Bovine insulin 58 %

A high quality enzyme immunoassay for specific quantification of human insulin in serum or plasma.

Kit format: 1 x 96 wells
Measurement range: 0.15 – 20 mU/L (0.0065 – 0.87 µg/L)

  • Calibrated against 1st International Reference Preparation 66/304 for human insulin
  • No cross-reactivity to C-peptide or Proinsulin des(31-32) or split (32-33)
  • Insignificant or no cross-reactivity to insulin analogs
  • CE/IVD labeled on select markets

Test Characteristics

  • Samples: Serum, plasma
  • Sample volume: 25 µL
  • Assay range: 0.15 – 20 mU/L
  • Detection limit: ≤ 0.15 mU/L (0.0065 µg/L)
  • Incubation: 1 h + 30 min
  • Detection method: absorbance

Specificity

  • C-peptide <0.01 %
  • Proinsulin <0.01 %
  • Proinsulin des (31-32) <0.5 %
  • Proinsulin split (32-33) <0.5 %
  • Proinsulin des (64-65) 98 %
  • Proinsulin split (65-66) 56 %
  • Insulin aspart 3 %
  • Insulin detemir n.d.
  • Insulin glargine 12 %
  • Insulin glulisine n.d.
  • Insulin lispro n.d.
  • IGF-I <0.02 %
  • IGF-II <0.02 %
  • Rat insulin 0.7 %
  • Mouse insulin 0.3 %
  • Porcine insulin 93 %
  • Ovine insulin 48 %
  • Bovine insulin 31 %

A high quality immunoassay for specific quantification of human C-peptide in serum, plasma or urine.

Kit format: 1×96 wells
Measurement range: 100 – 4000 pmol/L (0.302 – 12.1 µg/L)

  • Calibrated against International Reference Reagent IRR84/510 for human C-peptide
  • High specificity enables measurement of human C-peptide in mouse and rat models
  • Little or no cross-reactivity to human insulin or proinsulin
  • CE/IVD labeled on select markets

Test Characteristics

  • Samples: serum, EDTA and heparin plasma or urine
  • Sample volume: 25 µL
  • Assay range: 100-4000 pmol/L (0.3-12.0 ng/mL)
  • Detection limit: ≤ 25 pmol/L (0.076 μg/L)
  • Incubation: 1h + 1h + 15 min
  • Detection method: absorbance

Specificity

  • Insulin < 0.0006 %
  • Proinsulin 2 %
  • Proinsulin (Des 31-32) 3 %
  • Proinsulin (Split 32-33) 2 %
  • Proinsulin (Des 64-65) 74 %
  • Proinsulin (Split 65-66) 10 %

The Mercodia Ultrasensitive C-peptide ELISA is a high quality immunoassay for specific quantification of human C-peptide in serum, plasma, cell culture media or urine.

Kit format: 1 x 96 wells
Measurement range: 5 – 280 pmol/L (0.015 – 0.85 ng/mL)

  • High sensitivity, detetction limit < 2.5 pmol/L (0.0076 µg/L)
  • No or minimal cross-reactivity to insulin or proinsulin
  • Calibrated against International Reference Reagent IRR84/510 for human C-peptide
  • CE/IVD labeled on select markets

Test Characteristics

  • Samples: Serum, EDTA and heparin plasma as well as urine.
  • Sample volume: 50 µL
  • Assay range: 5 – 280 pmol/L (0.015 – 0.85 ng/mL)
  • Detection limit: 2.5 pmol/L (0.0076 µg/L)
  • Incubation: 1 h + 1 h + 30 min
  • Detection method: absorbance

Specificity

  • Insulin < 0.0006 %
  • Proinsulin 5 %
  • Proinsulin (Des 31-32) 3 %
  • Proinsulin (Split 32-33) 2 %
  • Proinsulin (Des 64-65) 74 %
  • Proinsulin (Split 65-66) 10 %

Mercodia Proinsulin ELISA provides a method for the quantitative determination of human proinsulin in serum or plasma.

Kit format: 1 x 96 wells
Measurement range: 3.3 – 130 pmol/L (0.03 – 1.18 µg/L)

  • Measures total proinsulin
  • Calibrated against International Reference Reagent IRR 84/611 for human proinsulin
  • Highly specific with no cross-reactivity to human insulin or C-peptide

Test Characteristics

  • Samples: Serum and plasma
  • Sample volume: 50 µL
  • Assay range: 3.3 – 130 pmol/L
  • Detection limit: 1.7 pmol/L
  • Incubation: 1 h + 1 h + 15 min
  • Detection method: absorbance

Specificity

  • Insulin < 0.03 %
  • C-peptide < 0.006 %
  • Proinsulin (Des 31-32) 95 %
  • Proinsulin (Split 32-33) 95 %
  • Proinsulin (Des 64-65) 84 %
  • Proinsulin (Split 65-66) 90 %
  • Porcine Proinsulin 4 %
  • Mouse Proinsulin I 3 %
  • Mouse Proinsulin II 16 %
  • Rat Proinsulin I 13 %
  • Rat Proinsulin II 10 %

The Mercodia Glucagon ELISA is a CE/IVD-labelled, highly specific and sensitive immunoassay for measuring glucagon in plasma and serum.

Kit format: 1 x 96 wells
Measurement range: 1.5 – 130 pmol/L (5 – 453 pg/mL)

  • Sample volume: 25 µL
  • No purification of samples
  • Sample types: Serum, EDTA Plasma, and cell-culture medium
  • Measurement range: 1.5 – 130 pmol/L (5.95 – 453pg/mL)
  • Detection limit: 0.75 pmol/L (2.61 pg/mL)
  • No cross-reactivity to glicentin, proglucagon 1-16, and glucagon 3-29
  • Highly appreciated and often cited assay
  • CE/IVD labeled on select markets

Test Characteristics

  • Samples: Serum, EDTA plasma and cell culture medium.
  • Sample volume: 25 µL
  • Assay range: 1.5-130 pmol/L (5.95-453pg/mL)
  • Detection limit: 0.75 pmol/L
  • Incubation: 18-22 h (overnight) + 1 h, 30 min
  • Detection method: absorbance

Specificity

  • Glicentin n.d
  • Oxyntomodulin 4.0 %
  • Proglucagon 1-61 n.d
  • Glucagon 3-29 n.d

The Mercodia Glicentin ELISA is a high quality immunoassay for the quantification of human glicentin in serum, plasma and cell culture media.

Kit format: 1×96 wells
Measurement range: 3 – 300 pmol/L (24 – 2400 pg/mL)

  • Highly specific, sensitive and rapid method for determination of glicentin
  • No cross-reactivity to circulating proglucagon derived gut peptides
  • Assay buffers and calibrators have been optimized to prevent degradation and aggregation of glicentin

Test Characteristics

  • Samples: Serum, EDTA plasma and cell culture media
  • Sample volume: 25 µL
  • Assay range: 3 – 300 pmol/L (24-2400 pg/mL)
  • Detection limit: 3 pmol/L (24 pg/mL)
  • Incubation: 1 h + 1 h + 15 min
  • Detection method: absorbance

Specificity

  • Glucagon N.D.
  • Oxyntomodulin N.D.
  • Mini-glucagon N.D.
  • GLP-1 N.D
  • GLP-2 N.D.
  • Rat glicentin 0.9 %
  • Mouse glicentin N.D

Mercodia Total GLP-1 NL-ELISA provides a chemiluminescent method for the quantitative determination of amidated GLP-1 (glucagon-like-peptide-1) in human serum or plasma samples.

Kit format: 1×96 wells
Measurement range: 0.9 – 940 pmol/L (2.8 – 2940 pg/mL)

  • 100% specificity to the predominating isoform, GLP-1 (9-36) amide
  • High sensitivity
  • Validated according to CLSI/FDA/EMA

Test Characteristics

  • Samples: Human serum and EDTA plasma
  • Sample volume: 25 µL
  • Assay range: 1 – 940 pmol/L
  • Detection limit: 0.65 pmol/L
  • Incubation: 2h + 15 min
  • Detection method: chemiluminescence

Specificity

  • GLP-1 (1-36) amide 88%
  • GLP-1 (7-36) amide 93%
  • GLP-1 (9-36) amide 100%
  • GLP-1 (1-37) n.d.
  • GLP-1 (7-37) n.d.
  • Glucagon n.d.
  • GIP n.d.
  • GLP-2 n.d.
  • Oxyntomodulin n.d.
  • Glicentin n.d.
  • Miniglucagon n.d.
  • MPGF n.d.
  • Liraglutide n.d.
  • Exenatide n.d.
  • Lixisenatide n.d.
  • Dulaglutide 0.07%

Mercodia Lispro NL-ELISA is designed to be an excellent assay for pharma use. It provides a chemiluminescent method for the quantitative determination of insulin lispro.

Kit format: 1 x 96 wells
Measurement range: 1 – 500 mU/L

  • Optimized for pharma use
  • No cross-reactivity to endogenous insulin or other insulin analogs e.g. glargine
  • No interference from insulin auto-antibodies
  • Fully validated for use with human and pig samples, partially validated for rat samples
  • Controls included in the kit

Test Characteristics

  • Samples: human and porcine serum, EDTA plasma and P-800 plasma
  • Sample volume: 10 µL
  • Assay range: 1 – 500 mU/L
  • Detection limit: 0.36 mU/L
  • Incubation: 1h + 1h + 10 min
  • Detection method: Chemiluminescence

Specificity

  • Human insulin N.D.
  • Human proinsulin N.D.
  • Glargine N.D.
  • Glargine M1 N.D.
  • Glargine M2 N.D.
  • Degludec N.D.
  • Determir N.D.
  • Insulin NPH N.D.
  • Aspart N.D.
  • Glulisine N.D.
  • Porcine insulin N.D.
  • Porcine proinsulin N.D.

The Mercodia Total GIP NL-ELISA provides a method for the quantitative determination of GIP in human samples.

Kit format: 1 x 96 wells
Measurement range: 2.7 – 1000 pmol/L

  • Measures both active GIP(1-42) and the inactive isoform GIP(3-42) in total
  • Broad measuring range
  • High sensitivity

Test Characteristics

  • Samples: Human serum and EDTA plasma
  • Sample volume: 25µL
  • Assay range: 2.7 – 1000 pmol/L
  • Detection limit: 1.62 pmol/L
  • Incubation: over night
  • Detection method: chemiluminescence

Specificity

  • GIP(1-42) 114-124%
  • GIP(3-42) 100%
  • GIP(1-30) n.d
  • GIP(3-30) n.d
  • Glucagon n.d
  • GLP-1(9-36)amide n.d
  • GLP-2 n.d
  • Oxyntomodulin n.d
  • Glicentin n.d
  • Miniglucagon n.d
  • Proglucagon 1-61 n.d
  • MPGF n.d
  • PACAP-38 n.d