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Mammalian Diabetes

Description Rat Insulin ELISA Ultrasensitive Rat Insulin ELISA High Range Rat Insulin ELISA
Code No. 10-1250-01
10-1250-10		 10-1251-01
10-1251-10		 10-1145-01
10-1145-10
No. of tests 96/10x96 96/10x96 96/10x96
Measured antigen Rat/mouse insulin Rat/mouse insulin Rat/mouse insulin
Standards ug/l 0, 0.15, 0.4, 1, 3, 5.5 0, 0.02, 0.05, 0.15 0.4, 1.0 0, 3.0, 7.5, 30.0, 75.0, 150.0
Calibration      
Incubations (mins) 120+15 120+15 120+15
Sample/sample volume serum/plasma 10ul serum/plasma 25ul serum/plasma 10ul
Sensitivity ≤0.15ug/l 1) ≤0.02ug/l 1.5 ug/l/
263 pmol/l
Precision intra/inter cv% <5.2%, <10.1% 1) <3% 1) <5% <5%/<5%
Hook >450 ug/l 450 ug/l

 576 ug/l
100.8nmol/l

Cross-reactions
  • Human insulin 167%
  • Insulin Lispro (Humalog®) 167%
  • Human proinsulin 75%
  • Human C-peptide <0.05%
  • IGF-I <0.02%
  • IGF-II <0.02%
  • Rat C-peptide <0.001%
  • Rat proinsulin 7%
  • Mouse insulin 75%
  • Porcine insulin 476%
  • Sheep insulin 179%
  • Bovine insulin 78%
  • No interference by lipaemic, icteric or haemolysed samples
  • insulin (mouse) 81%
  • insulin (human) 120%
  • insulin (Humalog®)120%
  • proinsulin (human) 71%
  • C-peptide (human) <0.05%
  • No interference by lipaemic, icteric or haemolysed samples
  • insulin (mouse) 81%
  • insulin (human) 120%
  • insulin (Humalog®) 120
  • proinsulin (human) 71% C-peptide (human)<0.05%
  • No interference by lipaemic, icteric or haemolysed samples
Description Porcine Insulin ELISA Ovine Insulin ELISA Bovine Insulin ELISA
Code No. 10-1200-01 10-1202-01 10-1201-01
No. of tests 96 96 96
Measured antigen Porcine insulin Ovine insulin Bovine insulin
Standards ug/l 0, 0.02, 0.05, 0.15, 0.5, 1.5 0, 0.05, 0.15, 0.5, 1.5, 3.0 0, 0.05, 0.15, 0.5, 1.5, 3.0
Calibration in-house reference in-house reference in-house reference
Incubations (mins) 120+15 120+15 120+15
Sample/sample volume Serum/plasma 25ul Serum/plasma 25ul Serum/plasma 25ul
Sensitivity <0.01ug/l <0.025ug/l <0.025ug/l
Precision intra/inter cv% <3.8% <3.9% <3.7% <6.5% <5.3% <7.0%
Hook >1000ug/l >1000ug/l >1000ug/l
Cross-reactions No interference by lipaemic, icteric or haemolysed samples No interference by lipaemic, icteric or haemolysed samples No interference by lipaemic, icteric or haemolysed samples
Description Mouse Insulin ELISA Ultrasensitive Mouse Insulin ELISA
Code No. 10-1247-01
10-1247-10		 10-1249-01
10-1249-10
No. of tests 96/10x96 96/10x96
Measured antigen Mouse Insulin Mouse Insulin
Standards ug/l 0, 0.188, 0.5, 1.25, 3.75, 6.9 0, 0.025, 0.063, 0.188, 0.5, 1.25
Incubation (mins) 120 + 15 120 + 15
Sample/sample volume serum/plasma 10ul serum/plasma 25ul
Sensitivity <0.2 ug/l < 0.025 ug/l
Precision intra/inter cv% <3.2%/<6% <3%/<5%
Hook >450 ug/l >450 ug/l
Cross-reactions No interference by lipaemic, icteric or haemolysed samples No interference by lipaemic, icteric or haemolysed samples
Description Rat C-peptide ELISA Rat/Mouse proinsulin ELISA
Code No. 10-1172-01 10-1232-01
No. of tests 96 96
Measured antigen Rat C-peptide Rat and Mouse proinsulin
Standards pmol/l c. 0 - 4000 (0 + 5 standards) c. 0 - 2000 (0 + 5 standards)
Incubation (mins) 60 + 60 + 15 120+30
Sample/sample volume serum/plasma 10ul serum/plasma 25ul
Sensitivity 3 pmol/l
Precision intra/inter cv% <4.3%/<7.3% <3.7%/<6.4%
Hook 400000pmol/l 90000pmol/l
Cross-reactions
  • Human C-peptide <0.001%
  • Rat proinsulin 4.55%
  • Rat insulin <0.01%
  • No interference by lipaemic, icteric or haemolysed samples
  • Human insulin <0.0000001%
  • Human proinsulin 2 - 10% (mean 5%)
  • Human C-peptide <0.000001%
  • Rat C-peptide <0.0000001%
  • Rat insulin <0.000004%
  • Mouse insulin <0.0003%
  • Mouse C-peptide <0.00007%
  • No interference by lipaemic, icteric or haemolysed samples
Description High Range Porcine Insulin ELISA Feline Insulin ELISA
Code No. 10-1223-01 10-1233-01
No. of tests 96 96
Measured antigen Porcine Insulin Feline Insulin
Standards c. 0 - 75 g/l (0 + 5 standards) 0, 5, 35, 100, 350, 700 ng/l
Incubation (mins) 60 + 15 120+30
Sample/sample volume serum/plasma 10ul serum/plasma 10ul
Sensitivity 0.313ug/l 5ng/l
Precision intra/inter cv% <2.3%/<5% <4.8%/<8.9%
Hook 11500 ug/l 27600 ug/l
Cross-reactions
  • Porcine C-peptide <0.001%
  • Porcine Proinsulin <0.2%
  • Human insulin 28%
  • Human Proinsulin <0.1%
  • Human C-peptide <0.01%
  • Aspart (NovoRapid) 0.7%
  • Detemir (Levemir) <0.0000002%
  • Glargin (Lantus) 5.4%
  • Lispro (Humalog) <0.0000006%
  • No interference by lipaemic, icteric or haemolysed samples
  • Aspart (NovoRapid®) 5.9%
  • Detemir (Levemir®) <0.008%
  • Glargin (Lantus®) 8.4%
  • Lispro (Humalog®) <0.00000002%
  • Glulisine (Apidra®) <0.0000007%
  • Letsulin® , Caninsulin® 57.4%
  • No interference by lipaemic, icteric or haemolysed samples
Description Canine Insulin ELISA Equine Insulin ELISA
Code No. 10-1203-01 10-1205-01
No. of tests 96 96
Measured antigen Canine Insulin Equine Insulin
Standards ug/l 0, 0.02, 0.05, 0.15, 0.50, 1.50 0, 0.02, 0.05, 0.15, 0.50, 1.50
Calibration in-house porcine insulin reference in-house porcine insulin reference
Incubation (mins) 120 + 15 120 + 15
Sample/sample volume serum/plasma 25ul serum/plasma 25ul
Sensitivity <0.01 ug/l <0.01 ug/l
Precision intra/inter cv% <4.5%/<6.2% <3.4%/<4.3%
Hook >1000ug/l >1000ug/l
Cross-reactions
  • porcine insulin 100%
  • porcine C-peptide <0.001%
  • porcine proinsulin <0.20%
  • human insulin 28%
  • human C-peptide <0.01%
  • human proinsulin <0.1%
  • NovoRapid 0.7%
  • Levemir <0.0000002%
  • Lantus 5.4%
  • Humalog <0.0000006%
  • No interference by lipaemic, icteric or haemolysed samples
  • porcine insulin 100%
  • porcine C-peptide <0.001%
  • porcine proinsulin <0.20%
  • human insulin 28%
  • human C-peptide <0.01%
  • human proinsulin <0.1%
  • NovoRapid 0.7%
  • Levemir <0.0000002%
  • Lantus 5.4%
  • Humalog <0.0000006%
  • No interference by lipaemic, icteric or haemolysed samples

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