Human Diabetes

Description	Insulin ELISA	Iso-Insulin ELISA	Ultrasensitive Insulin ELISA
Code No.	10-1113-01 10-1113-10	10-1128-01	10-1132-01
No. of tests	96/10x96	96	96
Measured antigen	human insulin	human insulin insulin lispro	human insulin
Standards mU/l	0, 3, 10, 30,100, 200	0, 3, 10, 30,100	0, 0.15, 1, 3, 10, 20
Calibration	1st IRP 66/304	1st IRP 66/304	1st IRP 66/304
Incubations (mins)	60+15	60+15	60+30
Sample/sample volume	serum/plasma 25ml	serum/plasma 25ml	serum/plasma 25ml
Sensitivity	<1 mU/L <6 pmol/L	1 mU/L 6 pmol/L	<0.07 mU/L <0.42 pmol/L
Precision intra/inter cv%	<4%, <4%	<4%, <4%	<6%, <4%
Hook	30000mU/L 180nmol/L	2000 mU/L 12 nmol/L	800000 mU/l 4800 nmol/l
Cross-reactions	C-peptide <0.01% proinsulin <0.01% insulin lispro <0.006% h-proinsulin des (64-65) 98% h-proinsulin split (65-66) 56% h-proinsulin des (31-32) <0.5% h-proinsulin split (32-33) <0.5%	C-peptide <0.1% proinsulin 54% insulin lispro 100% h-proinsulin des (64-65) 66% h-proinsulin split (65-66) 78% h-proinsulin des (31-32) 58% h-proinsulin split (32-33) 56%	C-peptide <0.01% proinsulin <0.01% insulin lispro <0.006% h-proinsulin des (64-65) 98% h-proinsulin split (65-66) 56% h-proinsulin des (31-32) <0.5% h-proinsulin split (32-33) <0.5%

Description	C-peptide ELISA specific	Ultrasensitive C-peptide ELISA	Proinsulin ELISA
Code No.	10-1136-01 10-1136-10	10-1141-01	10-1118-01
No. of tests	96/10x96	96	96
Measured antigen	human C-peptide	human C-peptide	human proinsulin
Standards p/mol/L	0, 100, 330, 1300, 2500, 4000	0, 5, 16, 60, 120, 230	0, 3.3, 11, 44, 132
Calibration	IRR 84/510	IRR 84/510	IRR 84/611
Incubations (mins)	60+60+15	60+60+30	60+60+15
Sample/sample volume	serum/plasma/urine 25ml	serum/plasma/urine 25ml	serum/plasma 50ml
Sensitivity	<15pmol/L <0.045ug/L	<1.5 pmol/L <0.0045ug/L	<0.5 pmol/L <0.0045ug/L
Precision intra/inter cv%	<5%, <5%	<4%, <6%	<5%, <6%
Hook	1750 nmol/L	36.6 nmol/L	80 nmol/lq
Cross-reactions	Insulin <0.0006% proinsulin <1.8% h-proinsulin des (64-65) 74% h-proinsulin split (65-66) 10% h-proinsulin des (31-32) 3% h-proinsulin split (32-33) 2%	Insulin <0.0006% proinsulin 3% h-proinsulin des (64-65) 74% h-proinsulin split (65-66) 10% h-proinsulin des (31-32) 3% h-proinsulin split (32-33) 2%	Insulin <0.03% C-peptide <0.006% h-proinsulin des (64-65) 84% h-proinsulin split (65-66) 90% h-proinsulin des (31-32) 95% h-proinsulin split (32-33) 95%

Description	Leptin ELISA	Adiponectin ELISA	Insulin Pre-Transplantation ELISA
Code No.	10-1199-10	10-1193-01	10-1222-01
No. of tests	96	96	96
Measured antigen	human leptin	human adiponectin	human insulin
Standards	0, 0.10, 0.25, 0.65, 1.50, 5.0 ng/ml	0, 5.0, 15.0, 50.0, 150.0, 300.0 ng/ml	0, 3.0, 10.0, 30.0, 100.0, 200.0, mU/l
Calibration	1st IS NIBSC Code 97/594	against validated commercial preparation	1st IRP 66/304
Incubation (mins)	120+15	60+60+15	15+15
Sample/sample volume	serum/plasma diluted 1/21	serum/plasma diluted 1/101	serum/plasma
Sensitivity	0.05 ng/ml	1.25 ng/ml	1.5 mU/l
Precision intra/inter cv%	<3% <5.2%	<3% <5.8%	<3% <4.5%
Hook	>100,000 ng/ml	none
Cross-reactions	CNTF ≤0.05% G-CSF ≤0.0004% IL-6 ≤0.0002% IL-11 ≤0.0008% IL-12 ≤0.0004% LIF ≤0.002% Oncostatin ≤0.0002% Rat Leptin 0.02% Mouse Leptin ≤0.001% Sheep Leptin 0.003%	C1q ≤0.007% TNF-a n.s	C-peptide <0.01% Proinsulin <0.01% Proinsulin des (64-65) 98% Proinsulin split (65-66) 56% Proinsulin des (31-32) <0.5% Proinsulin split (32-33) <0.5% Insulin Lispro (Humalog®, Eli Lilly) <0.006% Insulin aspart <0.006% IGF-I <0.02% IGF-II <0.02% Rat insulin 0.7% Mouse insulin 0.3% Porcine insulin 374% Sheep insulin 48% Bovine insulin 31%